Article
Changes in activation gating of IsK potassium currents brought about by mutations in the transmembrane sequence.
FEBS letters - 24 Oct 1994
Wilson G G, Sivaprasadarao A, Findlay J B, Wray D
Abstract excerpt
Expression of the rat kidney IsK protein in Xenopus oocytes produces slowly-activating potassium channel currents. We have investigated the relationship between structure and function of the single putative membrane-spanning domain using site-directed mutagenesis. Six mutants were constructed in...
Topics
- Amino Acid Sequence
- Animals
- Cell Membrane
- Ion Channel Gating
- Kidney
- Membrane Potentials
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oocytes
- Phenylalanine
- Potassium Channels
- Potassium Channels, Voltage-Gated
- Protein Structure, Secondary
- Rats
- Xenopus