Article
Tissue-type plasminogen activator domain-deletion mutant BM 06.022: modular stability, inhibitor binding, and activation cleavage.
Biochemistry - 4 Oct 1994
Hu C K, Kohnert U, Wilhelm O, Fischer S, Llinás M
Abstract excerpt
Recombinant BM 06.022 (M(r) 39,589) is a domain-deletion mutant of the human tissue-type plasminogen activator (tPA) structured by the kringle 2 and protease modules. Unfolding under various conditions was investigated via 1H-NMR spectroscopy by monitoring the well-resolved high-field methyl reso...
Topics
- Acids
- Amino Acid Chloromethyl Ketones
- Amino Acid Sequence
- Dansyl Compounds
- Enzyme Activation
- Enzyme Stability
- Hot Temperature
- Kringles
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Protein Denaturation
- Recombinant Proteins
- Sequence Deletion
- Tissue Plasminogen Activator