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Article

Converting tissue plasminogen activator to a zymogen: a regulatory triad of Asp-His-Ser.

Science (New York, N.Y.) - 15 Oct 1993

Madison E L, Kobe A, Gething M J, Sambrook J F, Goldsmith E J

Abstract excerpt

Unlike most serine proteases of the chymotrypsin family, tissue-type plasminogen activator (tPA) is secreted from cells as an active, single-chain enzyme with a catalytic efficiency only slightly lower than that of the proteolytically cleaved form. A zymogenic mutant of tPA has been engineered that displays a reduction in catalytic efficiency by a factor of 141 in the single-chain form while retaining full...

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Identifiers

PMID: 8211162
DOI: 10.1126/science.8211162

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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