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Article

Converting tissue plasminogen activator to a zymogen: a regulatory triad of Asp-His-Ser.

Science (New York, N.Y.) - 15 Oct 1993

Madison E L, Kobe A, Gething M J, Sambrook J F, Goldsmith E J

Abstract excerpt

Unlike most serine proteases of the chymotrypsin family, tissue-type plasminogen activator (tPA) is secreted from cells as an active, single-chain enzyme with a catalytic efficiency only slightly lower than that of the proteolytically cleaved form. A zymogenic mutant of tPA has been engineered th...

Topics

Amino Acid Sequence
Aspartic Acid
Base Sequence
Catalysis
Chymotrypsin
Enzyme Precursors
Histidine
Hydrogen Bonding
Kinetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasminogen

Plasminogen Activator Inhibitor 1

Serine

Tissue Plasminogen Activator

Source

Identifiers

PMID: 8211162
DOI: 10.1126/science.8211162

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8211162-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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