Article
Characterization of recombinant proteins: biochemical and biophysical criteria for the identity of native and renatured human tissue plasminogen activator and its mutants.
Biotechnology and applied biochemistry - 1 Aug 1994
Vogl T, Hinz H J
Abstract excerpt
Rapid progress in recombinant DNA techniques has led to the production of a great variety of native and modified proteins in bacteria which, however, frequently occur in non-native conformations or in aggregated form. Renaturation methods must necessarily include criteria for the confirmation of...
Topics
- Amino Acid Sequence
- Biophysical Phenomena
- Biophysics
- Humans
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Recombinant Proteins
- Structure-Activity Relationship
- Thermodynamics
- Tissue Plasminogen Activator