Characterization of recombinant proteins: biochemical and biophysical criteria for the identity of native and renatured human tissue plasminogen activator and its mutants.

Abstract excerpt

Rapid progress in recombinant DNA techniques has led to the production of a great variety of native and modified proteins in bacteria which, however, frequently occur in non-native conformations or in aggregated form. Renaturation methods must necessarily include criteria for the confirmation of the native structure and function of the refolded macromolecules. This Review describes some of the spectroscopic and...

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