Article
Physical separation of HLA-A alleles by denaturing high-performance liquid chromatography.
Tissue antigens - 1 Jun 2003
Etokebe G E, Opsahl M, Tveter A K, Lie B A, Thorsby E, Vartdal F, Spurkland A
Abstract excerpt
Genomic typing of polymorphic loci may be hampered by ambiguous typing results. Moreover, robust methods for simultaneous sequencing of two alleles present in a given sample may be difficult to establish. We used denaturing high-performance liquid chromatography (DHPLC) for physical separation of...
Topics
- Alleles
- Base Sequence
- Chromatography, High Pressure Liquid
- DNA
- DNA Primers
- Exons
- Feasibility Studies
- Gene Amplification
- HLA Antigens
- Heteroduplex Analysis
- Heterozygote
- Histocompatibility Testing
- Humans
- Nucleic Acid Denaturation
- Nucleic Acid Heteroduplexes
- Polymerase Chain Reaction
- Sequence Analysis, DNA