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Physical separation of HLA-A alleles by denaturing high-performance liquid chromatography.

Tissue antigens - 1 Jun 2003

Etokebe G E, Opsahl M, Tveter A K, Lie B A, Thorsby E, Vartdal F, Spurkland A

Abstract excerpt

Genomic typing of polymorphic loci may be hampered by ambiguous typing results. Moreover, robust methods for simultaneous sequencing of two alleles present in a given sample may be difficult to establish. We used denaturing high-performance liquid chromatography (DHPLC) for physical separation of HLA-A alleles before sequence-based genomic typing (SBT). Physical separation was achieved by resolution of...

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PMID: 12823768
DOI: 10.1034/j.1399-0039.2003.00058.x

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