Article
HLA class II DRB high resolution genotyping by pyrosequencing: comparison of group specific PCR and pyrosequencing primers.
Human immunology - 1 Feb 2004
Ringquist Steven, Alexander Angela M, Styche Alexis, Pecoraro Christopher, Rudert William A, Trucco Massimo
Abstract excerpt
Sequencing of alleles of the highly polymorphic, multiple loci HLA-DRB gene family was performed by pyrosequencing using purified DNA from the 11(th) International Histocompatibility Workshop human lymphoblastiod cell lines as well as genomic DNA isolated from blood samples obtained from healthy adult volunteers. Genomic DNA was prepared from donors whose blood had been stored either frozen or as dried blood...
Topics
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA
- DNA Primers
- Genotype
- HLA-DR Antigens
- HLA-DR beta-Chains
- HLA-DRB1 Chains
- HLA-DRB3 Chains
- HLA-DRB4 Chains