Article
Molecular analysis of HLA-DRB1*08/12 alleles: identification of two additional alleles.
Human immunology - 1 May 1992
Eberle M, Baxter-Lowe L A
Abstract excerpt
A nonradioactive oligotyping method that takes advantage of selective amplification using the polymerase chain reaction (PCR) and oligonucleotide probe hybridization was developed to distinguish all reported HLA-DRB1*08/12 alleles. Selective amplification was achieved using a primer complementary to the sequence encoding YSTGECY at positions 10-16 in the first hyperpolymorphic region (HPMR). This selective...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- HLA-DR Antigens
- HLA-DRB1 Chains
- Histocompatibility Antigens Class II
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction