Article
Mutational analysis defines a C-terminal tail domain of RAP1 essential for Telomeric silencing in Saccharomyces cerevisiae.
Genetics - 1 Dec 1994
Liu C, Mao X, Lustig A J
Abstract excerpt
Alleles specifically defective in telomeric silencing were generated by in vitro mutagenesis of the yeast RAP1 gene. The most severe phenotypes occur with three mutations in the C-terminal 28 amino acids. Two of the alleles are nonsense mutations resulting in truncated repressor/activator protein...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- DNA Mutational Analysis
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- GTP-Binding Proteins
- Gene Expression Regulation, Fungal
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Point Mutation
- Protein Structure, Tertiary
- Repressor Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Alignment
- Sequence Deletion