Article
Designing zinc-finger ADR1 mutants with altered specificity of DNA binding to T in UAS1 sequences.
Biochemistry - 14 Mar 1995
Taylor W E, Suruki H K, Lin A H, Naraghi-Arani P, Igarashi R Y, Younessian M, Katkus P, Vo N V
Abstract excerpt
Yeast ADR1 contains two Cys2,His2 zinc fingers needed for DNA binding to the upstream activation sequence UAS1, with bases T5T6G7-G8A9G10 in the ADH2 promoter. Potential DNA-contacting amino acid residues at -1, +3, and +6 in the alpha-helical domains of ADR1's fingers one and two include RHR-RLR...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- DNA Probes
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- Genes, Fungal
- Hydrogen Bonding
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors
- Zinc Fingers