A single amino acid substitution in zinc finger 2 of Adr1p changes its binding specificity at two positions in UAS1.

doi:10.1006/jmbi.1995.0410

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Article

A single amino acid substitution in zinc finger 2 of Adr1p changes its binding specificity at two positions in UAS1.

Journal of molecular biology - 4 Aug 1995

Cheng C, Young E T

Abstract excerpt

The two zinc fingers of the yeast transcription factor Adr1p recognize the 6 bp sequence TTG GAG site in which the first finger, a His-X3-His finger, recognizes the G-rich triplet (GAG) and the second zinc finger, a His-X4-His finger, recognizes the T-rich sequence (TTG). Mutations were introduced into the alpha-helical region of the second finger and the resultant mutant proteins were analyzed for DNA binding...

Topics

Amino Acid Sequence
Amino Acids
Arginine
Base Composition
Base Sequence
Binding Sites
DNA, Fungal
DNA-Binding Proteins
Fungal Proteins
Gene Expression
Genes, Reporter

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Guanine

Histidine

Leucine

Molecular Sequence Data

Mutagenesis, Site-Directed

Mutation

Protein Conformation

Source

Open in PubMed

Identifiers

PMID: 7643379
DOI: 10.1006/jmbi.1995.0410

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7643379-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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A single amino acid substitution in zinc finger 2 of Adr1p changes its binding specificity at two positions in UAS1.PMID 7643379Read ↗

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