A single amino acid change in a pathway-specific transcription factor results in differing degrees of constitutivity, hyperinducibility and derepression of several structural genes.

Abstract excerpt

unYc462 is a gain-of-function mutation in the purine catabolism positive regulatory gene of Aspergillus nidulans. This allele leads to a constitutive, hyperinducible and derepressed expression of a least three genes controlled by uaY, and this occurs at different levels depending on the target gene. The uaYc462 allele was mapped physically in relation to known loss-of-function alleles and sequenced. uaYc462 is a...

Topics

• Amino Acid Sequence
• Aspergillus nidulans
• Base Sequence
• DNA-Binding Proteins
• Gene Expression Regulation
• Molecular Sequence Data
• Mutation
• Saccharomyces cerevisiae
• Saccharomyces cerevisiae Proteins
• Transcription Factors