Article
Subtle hydrophobic interactions between the seventh residue of the zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA.
The EMBO journal - 1 Jul 1997
Ravagnani A, Gorfinkiel L, Langdon T, Diallinas G, Adjadj E, Demais S, Gorton D, Arst H N, Scazzocchio C
Abstract excerpt
A change of a universally conserved leucine to valine in the DNA-binding domain of the GATA factor AreA results in inability to activate some AreA-dependent promoters, including that of the uapA gene encoding a specific urate-xanthine permease. Some other AreA-dependent promoters become able to f...
Topics
- Aspergillus nidulans
- Binding Sites
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Membrane Transport Proteins
- Models, Molecular
- Molecular Structure
- Phenotype
- Point Mutation
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
- Repressor Proteins
- Transcription Factors