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Article

A simple method of HLA-DRB typing using enzymatically amplified DNA and immobilized probes on microtiter plate.

Human immunology - 1 Oct 1994

Kawai S, Maekawajiri S, Tokunaga K, Juji T, Yamane A

Abstract excerpt

We have developed a simple and economical method for HLA-DNA typing, called microtiter plate hybridization (PCR-MPH), which could replace standard PCR-SSO. This method is similar to that of an ELISA. Briefly, the PCR products labeled at the 5' termini with biotin were hybridized with probes immobilized on a microtiter well, and the bound PCR products were detected by streptavidin-conjugated enzymes followed by...

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PMID: 7860356
DOI: 10.1016/0198-8859(94)90004-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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