Article
A simple method of HLA-DRB typing using enzymatically amplified DNA and immobilized probes on microtiter plate.
Human immunology - 1 Oct 1994
Kawai S, Maekawajiri S, Tokunaga K, Juji T, Yamane A
Abstract excerpt
We have developed a simple and economical method for HLA-DNA typing, called microtiter plate hybridization (PCR-MPH), which could replace standard PCR-SSO. This method is similar to that of an ELISA. Briefly, the PCR products labeled at the 5' termini with biotin were hybridized with probes immobilized on a microtiter well, and the bound PCR products were detected by streptavidin-conjugated enzymes followed by...
Topics
- Base Sequence
- DNA, Single-Stranded
- Genotype
- HLA-DR Antigens
- HLA-DRB1 Chains
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction