Article
Oligonucleotide genotyping of HLA polymorphism on microtitre plates.
Lancet (London, England) - 10 Oct 1992
Cros P, Allibert P, Mandrand B, Tiercy J M, Mach B
Abstract excerpt
Molecular analysis of mutations and polymorphisms that are of medical importance requires both accuracy and simplicity. In organ transplantation there is a need for an HLA typing procedure that combines the remarkable accuracy of oligonucleotide genotyping with the simplicity of conventional serological typing. We describe a simple semiautomated method of HLA class II typing consisting of an oligonucleotide...
Topics
- Base Sequence
- Bone Marrow Transplantation
- DNA
- Genotype
- HLA-DR Antigens
- Haplotypes
- Histocompatibility Testing
- Humans
- Kidney Transplantation
- Leukemia
- Molecular Sequence Data
- Nucleic Acid Hybridization