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Article

Rapid DNA typing utilizing immobilized oligonucleotide probe and a nonradioactive detection system. Application to HLA-DR typing of the Japanese population.

Journal of immunological methods - 2 Oct 1992

Abe A, Kitagawa M, Ikuta Y, Miyakoshi S, Danbara H, Kashiwagi N, Obata F

Abstract excerpt

We established a rapid and simple method of HLA-DR genotyping, and applied it for analysis of the Japanese population. Our method includes rapid preparation of DNA samples from buccal mucosa, incorporation of biotin-dATP into DRB genes during amplification by the polymerase chain reaction, hybridization with sequence-specific oligonucleotide (SSO) probes immobilized on nylon membranes via poly (dT) tails, and...

Topics

Base Sequence
Genes, MHC Class II
Genotype
HLA-DR Antigens
Humans
Molecular Sequence Data
Mouth Mucosa
Oligonucleotide Probes
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1401953
DOI: 10.1016/0022-1759(92)90193-w

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1401953-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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