Article
A polymerase chain reaction-sequence-specific oligonucleotide procedure for HLA class II typing using biotin- and digoxigenin-labeled probes simultaneously in hybridization.
Human immunology - 1 Jan 1994
Chen D F, Endres W, Meyer S A, Stangel W
Abstract excerpt
To simplify PCR-SSO HLA-DRB generic typing, we labeled eight of 15 oligonucleotide probes with DIG and the others with biotin, and hybridized each dot blot with both a biotin-labeled probe and a DIG-labeled probe simultaneously. We chose oligonucleotide pairs which require the same hybridization and stringent washing conditions and do not compete with each other during hybridization. After incubation with a...
Topics
- Base Sequence
- Biotin
- Cell Line
- DNA
- Digoxigenin
- Genotype
- HLA-DR Antigens
- Humans
- Immunoblotting
- Molecular Sequence Data
- Nucleic Acid Hybridization