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Article

Dideoxy fingerprinting of low-level nucleotide variation in mitochondrial DNA of the human blood fluke, Schistosoma japonicum.

Electrophoresis - 1 Oct 1999

Zhu X, Bøgh H, Gasser R B

Abstract excerpt

Dideoxy fingerprinting (ddF) is an efficient method for the detection of sequence changes in polymerase chain reaction (PCR)-amplified DNA fragments. It is a hybrid between single-strand conformation polymorphism (SSCP) and dideoxy sequencing, employing only one dideoxynucleotide in the reaction. We report the application of ddF for the display of low-level nucleotide variation in the mitochondrial (mt) NADH...

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PMID: 10546814
DOI: 10.1002/(SICI)1522-2683(19991001)20:14<2830::AID-ELPS2830>3.0.CO;2-2

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