Template-directed dye-terminator incorporation (TDI) assay: a homogeneous DNA diagnostic method based on fluorescence resonance energy transfer.

doi:10.1093/nar/25.2.347

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Article

Template-directed dye-terminator incorporation (TDI) assay: a homogeneous DNA diagnostic method based on fluorescence resonance energy transfer.

Nucleic acids research - 15 Jan 1997

Chen X, Kwok P Y

Abstract excerpt

A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence resonance energy transfer is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a 5'-fluorescein-labeled primer (designed to hybridize...

Topics

Alleles
DNA
DNA Primers
Electrophoresis, Polyacrylamide Gel
Fluorescein
Fluoresceins
Fluorescent Dyes
Genetic Markers
Genotype
Polymerase Chain Reaction
Polymorphism, Genetic
Spectrometry, Fluorescence

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