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Article

Template-directed dye-terminator incorporation (TDI) assay: a homogeneous DNA diagnostic method based on fluorescence resonance energy transfer.

Nucleic acids research - 15 Jan 1997

Chen X, Kwok P Y

Abstract excerpt

A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence resonance energy transfer is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a 5'-fluorescein-labeled primer (designed to hybridize to the DNA template adjacent to the polymorphic site) in the presence of allelic dye-labeled dideoxyribonucleoside...

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Identifiers

PMID: 9016564
DOI: 10.1093/nar/25.2.347
PMCID: PMC146426

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