Article
Parameters affecting the sensitivities of dideoxy fingerprinting and SSCP.
PCR methods and applications - 1 Oct 1994
Liu Q, Sommer S S
Abstract excerpt
The goals of the present experiments are (1) to improve dideoxy fingerprinting (ddF) and (2) to utilize ddF as a tool to evaluate the relative merits of different conditions for single-strand conformation polymorphism (SSCP). ddF is performed by electrophoresing one dideoxy termination reaction through a nondenaturing gel. The ddF pattern can be divided into a "dideoxy component" and an "SSCP component". If...
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