Parameters affecting the sensitivities of dideoxy fingerprinting and SSCP.

doi:10.1101/gr.4.2.97

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Article

Parameters affecting the sensitivities of dideoxy fingerprinting and SSCP.

PCR methods and applications - 1 Oct 1994

Liu Q, Sommer S S

Abstract excerpt

The goals of the present experiments are (1) to improve dideoxy fingerprinting (ddF) and (2) to utilize ddF as a tool to evaluate the relative merits of different conditions for single-strand conformation polymorphism (SSCP). ddF is performed by electrophoresing one dideoxy termination reaction t...

Topics

Base Sequence
DNA
DNA Fingerprinting
Deoxycytosine Nucleotides
Dideoxynucleotides
Electrophoresis, Polyacrylamide Gel
Factor IX
Humans
Molecular Sequence Data
Mutation
Polymorphism, Single-Stranded Conformational
Temperature

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