Article
Fluorescence-based directed termination PCR: direct mutation characterization without sequencing.
Nucleic acids research - 15 Feb 2001
Chen J Z, Smith L, Pfeifer G P, Holmquist G P
Abstract excerpt
We describe a fluorescence-based directed termination PCR (fluorescent DT-PCR) that allows accurate determination of actual sequence changes without dideoxy DNA sequencing. This is achieved using near infrared dye-labeled primers and performing two PCR reactions under low and unbalanced dNTP concentrations. Visualization of resulting termination fragments is accomplished with a dual dye Li-cor DNA sequencer. As...
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