Article
CRISPR/Cas9/sgRNA-mediated targeted gene modification confirms the cause-effect relationship between gyrA mutation and quinolone resistance in Escherichia coli.
FEMS microbiology letters - 1 Jul 2018
Qiu Haixiang, Gong Jiansen, Butaye Patrick, Lu Guangwu, Huang Ke, Zhu Guoqiang, Zhang Jilei, Hathcock Terri, Cheng Darong, Wang Chengming
Abstract excerpt
Quinolones are broad-spectrum antibiotics that have been used for decades in treating bacterial infections in humans and animals, and subsequently bacterial resistance to these agents has increased. While studies indicated the relationship between gyrA mutations and bacterial resistance to quinolones, CRISPR/Cas9 was used in this study to investigate causal role of gyrA mutation in the quinolone resistance. In...
Topics
- Amino Acid Motifs
- Animals
- Anti-Bacterial Agents
- CRISPR-Cas Systems
- Chickens
- Ciprofloxacin
- DNA Gyrase
- Drug Resistance, Bacterial
- Escherichia coli
- Escherichia coli Infections