Article
Alterations in the DNA topoisomerase IV grlA gene responsible for quinolone resistance in Staphylococcus aureus.
Antimicrobial agents and chemotherapy - 1 May 1996
Yamagishi J, Kojima T, Oyamada Y, Fujimoto K, Hattori H, Nakamura S, Inoue M
Abstract excerpt
A 4.2-kb DNA fragment conferring quinolone resistance was cloned from a quinolone-resistant clinical isolate of Staphylococcus aureus and was shown to possess a part of the grlB gene and a mutated grlA gene. S-80-->F and E-84-->K mutations in the grlA gene product were responsible for the quinolone resistance. The mutated grlA genes responsible for quinolone resistance were dominant over the wild-type allele,...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Topoisomerase IV
- DNA Topoisomerases, Type II
- Drug Resistance, Microbial
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Open Reading Frames