Article
Homogeneous fluorescence detection method for human leukocyte antigen-DR typing following polymerase chain reaction amplification with sequence-specific primer.
Analytical biochemistry - 1 Sept 1994
Okamoto N, Lee A, Kano T, Lee T D
Abstract excerpt
A fluorescent homogeneous method for the detection of sequence-specific amplification of human leukocyte antigen (HLA) alleles has been developed. In this approach, polymerase chain reaction sequence-specific primers (PCR-SSP) are used to amplify DRB1, DRB3, and DRB4 alleles. Lambda exonuclease and Exonuclease I are added to reduce background by digesting template DNA, partial primer dimer, and primer. PCR...
Topics
- Alleles
- Base Sequence
- DNA
- DNA Primers
- DNA Repair Enzymes
- Exodeoxyribonucleases
- Fluorescent Dyes
- HLA-DR Antigens
- Histocompatibility Testing
- Humans
- Leukocytes