Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes.

doi:10.1016/S0002-9440(10)65650-7

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Article

Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes.

The American journal of pathology - 1 Oct 1998

Bernard P S, Ajioka R S, Kushner J P, Wittwer C T

Abstract excerpt

Multiplex polymerase chain reaction amplification and genotyping by fluorescent probe melting temperature (Tm) was used to simultaneously detect multiple variants in the hereditary hemochromatosis gene. Homogenous real-time analysis by fluorescent melting curves has previously been used to genoty...

Topics

Alleles
DNA
DNA Primers
DNA Probes
Genes
Genotype
Hemochromatosis
Heteroduplex Analysis
Humans
In Situ Hybridization, Fluorescence
Point Mutation
Polymerase Chain Reaction
Polymorphism, Genetic

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