Solid-phase direct DNA sequencing of allele-specific polymerase chain reaction-amplified HLA-DR genes.

Article

Solid-phase direct DNA sequencing of allele-specific polymerase chain reaction-amplified HLA-DR genes.

BioTechniques - 1 Jan 1991

Kaneoka H, Lee D R, Hsu K C, Sharp G C, Hoffman R W

Abstract excerpt

We describe in this report a new strategy to directly sequence polymerase chain reaction-amplified human leucocyte antigen DRB genes using biotinylated allele-specific synthetic oligonucleotide primers coupled to streptavidin-coated magnetic beads. The use of allele-specific primers in the polyme...

Topics

Alleles
Base Sequence
Cloning, Molecular
Genes
HLA-DR Antigens
Humans
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction
Polymorphism, Genetic

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