Article
Solid-phase direct DNA sequencing of allele-specific polymerase chain reaction-amplified HLA-DR genes.
BioTechniques - 1 Jan 1991
Kaneoka H, Lee D R, Hsu K C, Sharp G C, Hoffman R W
Abstract excerpt
We describe in this report a new strategy to directly sequence polymerase chain reaction-amplified human leucocyte antigen DRB genes using biotinylated allele-specific synthetic oligonucleotide primers coupled to streptavidin-coated magnetic beads. The use of allele-specific primers in the polymerase chain reaction allows for selective amplification of DNA from one haplotype, which when combined with the direct...
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