Article
Fluorotyping of HLA-DRB by sequence-specific priming and fluorogenic probing.
Tissue antigens - 1 Mar 1999
Albis-Camps M, Blasczyk R
Abstract excerpt
Similar to our recently described HLA-A and -C fluorotyping strategies, the aim of this study was to develop a sequence-specific primed polymerase chain reaction (PCR-SSP)-based fluorotyping method for HLA-DRB. Applying the fluorogenic 5' nuclease assay, it is possible to increase the sample throughput rate by abolishing all labor-intensive post-amplification steps. Additionally, problems related to contamination...
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