Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Semiautomated HLA-DQB1 typing by fluorescent dye photometry of amplified DNA on microtiter plates.

Human immunology - 1 Jan 1994

Bein G, Haase D, Schult J, Eiermann T H, Kirchner H

Abstract excerpt

We report a simple semiautomated HLA class II typing method that suits the demands of a 24-hour-duty transplantation service for preorgan retrieval donor typing. The procedure consists of sequence-specific amplification of HLA alleles by the polymerase chain reaction (nested PCR-SSP) followed by...

Topics

Alleles
Base Sequence
Cell Line
Cell Transformation, Viral
DNA
DNA Primers
Fluorescent Dyes
Genotype
HLA-DQ Antigens
HLA-DQ beta-Chains
Humans
Molecular Sequence Data
Photometry

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Sensitivity and Specificity

Source

Identifiers

PMID: 7910159
DOI: 10.1016/0198-8859(94)90094-9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7910159-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Semiautomated HLA-DQB1 typing by fluorescent dye photometry of amplified DNA on microtiter plates.PMID 7910159Read ↗

Oligonucleotide genotyping of HLA polymorphism on microtitre plates.Semantic proximity Read ↗

A low-cost, sensitive and specific PCR-based tool for rapid clinical detection of HLA-B*35 alleles associated with delayed drug hypersensitivity reactions.Semantic proximity Read ↗

Phototyping: comprehensive DNA typing for HLA‐A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence‐specific primers (PCR‐SSP)Semantic proximity Read ↗

DNA typing with digoxigenin-11-dideoxyuridinetriphosphate-labelled oligonucleotide probes enables non-radioactive analysis using a dual detection system: application for screening HLA-DQA1 polymorphisms.Semantic proximity Read ↗

How can we reduce costs of solid-phase multiplex-bead assays used to determine anti-HLA antibodies?Semantic proximity Read ↗

Bead‐by‐bead normalization of single antigen assays: A necessary step for accurate detection of weak anti‐HLA antibodiesSemantic proximity Read ↗

Time-resolved fluorometry based sandwich hybridisation assay for HLA-DQA1 typing.Semantic proximity Read ↗

High accuracy of HLA-B*27 genotyping by allele-specific real-time polymerase chain reaction in a heterogeneous population compared to flow cytometry and single nucleotide polymorphism detection assays.Semantic proximity Read ↗

Rapid allelic discrimination from real-time DNA amplification.Semantic proximity Read ↗

Sequence-specific priming and exonuclease-released fluorescence detection of HLA-DQB1 alleles.Semantic proximity Read ↗

Single-step allele-specific polymerase chain reaction HLA-DQ genotyping using ARMS primers.Semantic proximity Read ↗

Template-directed dye-terminator incorporation (TDI) assay: a homogeneous DNA diagnostic method based on fluorescence resonance energy transfer.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.