Article
[Reversal of malignant phenotype of human hepatoma cells by antisense c-ets-2, c-myc and N-ras].
Zhonghua zhong liu za zhi [Chinese journal of oncology] - 1 Jul 1994
Huang X M, Wei S G, Wang L F
Abstract excerpt
A recombinant retroviral vector was constructed which expressed antisense RNA of c-ets-2, c-myc and N-ras. The pseudotype virus was packaged and rescued by transfection in PA317 cells and used to infect human hepatoma cell line SMMC-7721. After selection with G418, resistant colonies were obtained. Stable integration of retrovirus in infectants was shown by Southern hybridization of genomic DNA and the presence...
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