Article
[Reversal of malignant cell phenotype of human pancreatic adenocarcinoma cell line by recombinant retroviral vector expressing antisense Ki-ras].
Zhonghua bing li xue za zhi = Chinese journal of pathology - 1 Oct 1995
Li H, Wang Z, Liu T
Abstract excerpt
A recombinant retroviral vector expressing antisense Ki-ras was constructed. The recombinant virus DNA was packaged with packaging cell line PA317 cells by the calcium phosphate coprecipitation method. The virus supernatant was used to infect human pancreatic carcinoma cell line PC-2. After selection with Puromycin, resistant colonies were obtained. Stable expressions of retrovirus in both PA317 and PC-2 cells...
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