Article
The mcm2-1 mutation of yeast causes DNA damage with a RAD9 requirement for repair.
Current genetics - 1 Jan 1995
Ray A, Sinha P
Abstract excerpt
The minichromosome maintenance mutation, mcm2-1, has been found to synthesize damaged DNA at 35 degrees C. Growth at this temperature rendered the mutant strain more sensitive to killing by ultraviolet irradiation. DNA damage could also be detected by pulsed-field gel electrophoresis, where a higher fraction of the DNA loaded was retained in the inserts at the wells. During the exponential phase of growth at this...
Topics
- Cell Cycle
- Cell Cycle Proteins
- Chromosomal Proteins, Non-Histone
- DNA Damage
- DNA Repair
- DNA Replication
- DNA, Fungal
- Electrophoresis, Gel, Pulsed-Field
- Fungal Proteins
- Genetic Vectors
- Mutation