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Article

Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.

Molecular and cellular biology - 1 Dec 2012

Sabatinos Sarah A, Green Marc D, Forsburg Susan L

Abstract excerpt

Hydroxyurea (HU) treatment activates the intra-S phase checkpoint proteins Cds1 and Mrc1 to prevent replication fork collapse. We found that prolonged DNA synthesis occurs in cds1Δ and mrc1Δ checkpoint mutants in the presence of HU and continues after release. This is coincident with increased DNA damage measured by phosphorylated histone H2A in whole cells during release. High-resolution live-cell imaging shows...

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Identifiers

PMID: 23045396
DOI: 10.1128/MCB.01060-12
PMCID: PMC3510540

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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