Article
Overexpression of the Shigella flexneri genes coding for DNA topoisomerase IV compensates for loss of DNA topoisomerase I: effect on virulence gene expression.
Molecular microbiology - 1 Feb 1995
McNairn E, Ni Bhriain N, Dorman C J
Abstract excerpt
Introducing the Escherichia coli topA20::Tn10 allele to Shigella flexneri results in osmotic sensitivity, a reduced growth rate, an increase in reporter plasmid supercoiling (all common to the E. coli mutants), an inability to grow on MacConkey agar and a loss of virulence gene expression. E. col...
Topics
- Alleles
- Bacterial Proteins
- Cloning, Molecular
- DNA Topoisomerase IV
- DNA Topoisomerases, Type I
- DNA, Bacterial
- DNA, Superhelical
- Enzyme Induction
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genetic Complementation Test
- Plasmids
- Salmonella typhimurium
- Shigella flexneri
- Virulence