Article
Roles of topoisomerases in maintaining steady-state DNA supercoiling in Escherichia coli.
The Journal of biological chemistry - 17 Mar 2000
Zechiedrich E L, Khodursky A B, Bachellier S, Schneider R, Chen D, Lilley D M, Cozzarelli N R
Abstract excerpt
DNA supercoiling is essential for bacterial cell survival. We demonstrated that DNA topoisomerase IV, acting in concert with topoisomerase I and gyrase, makes an important contribution to the steady-state level of supercoiling in Escherichia coli. Following inhibition of gyrase, topoisomerase IV alone relaxed plasmid DNA to a final supercoiling density (sigma) of -0.015 at an initial rate of 0.8 links min(-1)....
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