Influence of DNA supercoiling on the loss of culturability of Escherichia coli cells incubated in seawater.

doi:10.1111/j.1365-294x.1992.tb00174.x

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Article

Influence of DNA supercoiling on the loss of culturability of Escherichia coli cells incubated in seawater.

Molecular ecology - 1 Oct 1992

Gauthier M J, Labedan B, Breittmayer V A

Abstract excerpt

The relationship between the loss of culturability of Escherichia coli cells in seawater and the DNA supercoiling level of a reporter plasmid (pUC8) have been studied under different experimental conditions. Transfer to seawater of cells grown at low osmolarity decreased their ability to grow wit...

Topics

Culture Media
DNA Topoisomerases, Type I
DNA Topoisomerases, Type II
DNA, Bacterial
DNA, Superhelical
Ecosystem
Escherichia coli
Mutation
Osmolar Concentration
Plasmids
Seawater
Topoisomerase II Inhibitors
Water Microbiology

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