Intragenomic complementation of a 3AB mutant in dicistronic polioviruses.

doi:10.1006/viro.1995.1263

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Article

Intragenomic complementation of a 3AB mutant in dicistronic polioviruses.

Virology - 1 Jun 1995

Cao X, Wimmer E

Abstract excerpt

We report the construction of a poliovirus genome [pPVM-VPg(3F4A)] harboring a double mutation in VPg. This mutant, in which the tyrosine and the threonine at residues 3 and 4 of the VPg region were replaced by phenylalanine and alanine, respectively, is lethal, that is, all RNA synthesis was abo...

Topics

Amino Acid Sequence
Base Sequence
DNA Primers
Genes, Viral
Genetic Complementation Test
Genome, Viral
Genotype
HeLa Cells
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed
Open Reading Frames
Phenotype
Point Mutation
Poliovirus
Polymerase Chain Reaction
Protein Biosynthesis
RNA, Viral

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