Article
22 genes from chromosome 17q21: cloning, sequencing, and characterization of mutations in breast cancer families and tumors.
Genomics - 1 Jan 1995
Friedman L S, Ostermeyer E A, Lynch E D, Welcsh P, Szabo C I, Meza J E, Anderson L A, Dowd P, Lee M K, Rowell S E
Abstract excerpt
In our effort to identify BRCA1, 22 genes were cloned from a 1-Mb region of chromosome 17q21 defined by meiotic recombinants in families with inherited breast and/or ovarian cancer. Subsequent discovery of another meiotic recombinant narrowed the region to approximately 650 kb. Genes were cloned from fibroblast and ovarian cDNA libraries by direct screening with YACs and cosmids. The more than 400 cDNA clones so...
Topics
- BRCA1 Protein
- Base Sequence
- Breast
- Breast Neoplasms
- Chromosome Mapping
- Chromosomes, Artificial, Yeast
- Chromosomes, Human, Pair 17
- Cloning, Molecular
- DNA Primers
- DNA Transposable Elements