Article
Secretion by Saccharomyces cerevisiae of rat apolipoprotein E as a fusion to Mucor rennin.
Applied microbiology and biotechnology - 1 Mar 1995
Nomura N, Yamada H, Matsubara N, Horinouchi S, Beppu T
Abstract excerpt
As the first step for production of rat apolipoprotein E (rApoE) in Saccharomyces cerevisiae, the rApoE cDNA was cloned and its nucleotide sequence was determined. When the intact rApoE gene including the presequence-encoding region was expressed under the control of the yeast GAL7 promoter, no p...
Topics
- Amino Acid Sequence
- Animals
- Apolipoproteins E
- Aspartic Acid Endopeptidases
- Base Sequence
- Chymosin
- Cloning, Molecular
- DNA, Complementary
- Genes, Fungal
- Genetic Vectors
- Glycosylation
- Molecular Sequence Data
- Mucor
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Rats
- Rats, Sprague-Dawley