Article
Secretion by Saccharomyces cerevisiae of rat apolipoprotein E as a fusion to Mucor rennin.
Applied microbiology and biotechnology - 1 Mar 1995
Nomura N, Yamada H, Matsubara N, Horinouchi S, Beppu T
Abstract excerpt
As the first step for production of rat apolipoprotein E (rApoE) in Saccharomyces cerevisiae, the rApoE cDNA was cloned and its nucleotide sequence was determined. When the intact rApoE gene including the presequence-encoding region was expressed under the control of the yeast GAL7 promoter, no protein immunoreactive with anti-rApoE antibody was detected either in the culture medium or inside the cells. For the...
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