Article
Protein Engineering Approaches to Enhance Fungal Laccase Production in S. cerevisiae.
International journal of molecular sciences - 25 Jan 2021
Aza Pablo, de Salas Felipe, Molpeceres Gonzalo, Rodríguez-Escribano David, de la Fuente Iñigo, Camarero Susana
Abstract excerpt
Laccases secreted by saprotrophic basidiomycete fungi are versatile biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the sole requirement. Saccharomyces cerevisiae is a preferred host for engineering fungal laccases. To assist the difficult secretion of active enzymes by yeast, the native signal peptide is usually replaced by the preproleader of S. cerevisiae alfa mating factor...
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