Article
Introduction of an N-glycosylation site increases secretion of heterologous proteins in yeasts.
Applied and environmental microbiology - 1 Nov 2000
Sagt C M, Kleizen B, Verwaal R, de Jong M D, Müller W H, Smits A, Visser C, Boonstra J, Verkleij A J, Verrips C T
Abstract excerpt
Saccharomyces cerevisiae is often used to produce heterologous proteins that are preferentially secreted to increase economic feasibility. We used N-glycosylation as a tool to enhance protein secretion. Secretion of cutinase, a lipase, and llama V(HH) antibody fragments by S. cerevisiae or Pichia pastoris improved following the introduction of an N-glycosylation site. When we introduced an N-glycosylation...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.