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Article

Expression of rat cathepsin D cDNA in Saccharomyces cerevisiae: implications for intracellular targeting of cathepsin D to vacuoles.

Journal of biochemistry - 1 Jul 1995

Nishimura Y, Takeshima H, Sakaguchi M, Mihara K, Omura T, Kato K, Himeno M

Abstract excerpt

To investigate the intracellular transport mechanisms of lysosomal cathepsin D in yeast cells, we produced cathepsin D in Saccharomyces cerevisiae by placing the coding region under the control of the promoter of the yeast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Immunoblotting analysis by the use of an antibody specific for rat cathepsin D coding sequence produced an intermediate species which had...

Topics

Animals
Biological Transport
Cathepsin D
DNA, Complementary
Fluorescent Antibody Technique, Indirect
Gene Deletion
Glycosylation
Mutation
Promoter Regions, Genetic
Rats
Recombinant Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces cerevisiae

Tunicamycin

Vacuoles

Source

Identifiers

PMID: 8537307
DOI: 10.1093/oxfordjournals.jbchem.a124873

Genes

GAPDH

Provenance

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