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Article

Purification of Saccharomyces cerevisiae RNase H(70) and identification of the corresponding gene.

FEBS letters - 7 May 1999

Frank P, Braunshofer-Reiter C, Karwan A, Grimm R, Wintersberger U

Abstract excerpt

We purified Saccharomyces cerevisiae RNase H(70) to homogeneity, using an optimized chromatographic purification procedure. Renaturation gel assay assigned RNase H activity to a 70 kDa polypeptide. Sequencing of tryptic peptides identified the open reading frame YGR276c on chromosome VII of the S. cerevisiae genome as the corresponding gene, which encodes a putative polypeptide of molecular mass of 62849. We...

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PMID: 10359084
DOI: 10.1016/s0014-5793(99)00512-8

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