Expression and secretion of bovine beta-lactoglobulin in Saccharomyces cerevisiae.

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Article

Expression and secretion of bovine beta-lactoglobulin in Saccharomyces cerevisiae.

Agricultural and biological chemistry - 1 Dec 1990

Totsuka M, Katakura Y, Shimizu M, Kumagai I, Miura K, Kaminogawa S

Abstract excerpt

A bovine beta-lactoglobulin (beta-LG) was expressed in Saccharomyces cerevisiae carrying bovine pre-beta-LG cDNA and secreted into its growth medium. The expression plasmid was constructed by inserting the whole coding region of the cDNA encoding pre-beta-LG between the promoter and terminator of...

Topics

Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Cattle
Cloning, Molecular
DNA, Fungal
Enzyme-Linked Immunosorbent Assay
Gene Library
Lactoglobulins
Mammary Glands, Animal
Molecular Sequence Data
Mutation
Recombinant Proteins
Restriction Mapping
Saccharomyces cerevisiae

Source

Open in PubMed

Identifiers

PMID: 1368635

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1368635-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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