Article
Expression and secretion of bovine beta-lactoglobulin in Saccharomyces cerevisiae.
Agricultural and biological chemistry - 1 Dec 1990
Totsuka M, Katakura Y, Shimizu M, Kumagai I, Miura K, Kaminogawa S
Abstract excerpt
A bovine beta-lactoglobulin (beta-LG) was expressed in Saccharomyces cerevisiae carrying bovine pre-beta-LG cDNA and secreted into its growth medium. The expression plasmid was constructed by inserting the whole coding region of the cDNA encoding pre-beta-LG between the promoter and terminator of the yeast glyceraldehyde 3-phosphate dehydrogenase gene of pYG100, a yeast expression vector. In the supernatant of...
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