Article
A new signal peptide useful for secretion of heterologous proteins from yeast and its application for synthesis of hirudin.
Gene - 2 Jan 1992
Achstetter T, Nguyen-Juilleret M, Findeli A, Merkamm M, Lemoine Y
Abstract excerpt
The BGL2 gene from Saccharomyces cerevisiae encodes a beta-glucanase which is localized to the yeast cell wall. The ability of a 23-amino acid (aa) signal peptide derived from the BGL2 gene to direct a heterologous protein to the secretory pathway of yeast has been compared to that of the MF alpha 1-encoded signal peptide in a series of gene fusions. As a model protein, the leech anticoagulant, recombinant...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Deletion
- Genes, Fungal
- Genetic Variation
- Genetic Vectors
- Hirudins
- Molecular Sequence Data
- Plasmids
- Protein Processing, Post-Translational
- Protein Sorting Signals