Alpha-factor-leader-directed secretion of recombinant human-insulin-like growth factor I from Saccharomyces cerevisiae. Precursor formation and processing in the yeast secretory pathway.

doi:10.1111/j.1432-1033.1991.tb16063.x

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Article

Alpha-factor-leader-directed secretion of recombinant human-insulin-like growth factor I from Saccharomyces cerevisiae. Precursor formation and processing in the yeast secretory pathway.

European journal of biochemistry - 15 Jun 1991

Steube K, Chaudhuri B, Märki W, Merryweather J P, Heim J

Abstract excerpt

A synthetic gene coding for human-insulin-like growth factor I (IGFI) was fused to the leader sequence of yeast prepro-alpha-factor and expressed in Saccharomyces cerevisiae under the control of a glyceraldehyde-3-phosphate dehydrogenase promoter fragment. Recombinant IGFI was found inside yeast cells and secreted into the medium. The secreted IGFI migrated on SDS gels with the same electrophoretic mobility as...

Topics

Chromatography, High Pressure Liquid
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Fungal Proteins
Genetic Variation
Glyceraldehyde-3-Phosphate Dehydrogenases
Humans
Insulin-Like Growth Factor I
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Promoter Regions, Genetic

Protein Precursors

Recombinant Proteins

Restriction Mapping

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Spheroplasts

Source

Open in PubMed

Identifiers

PMID: 2050146
DOI: 10.1111/j.1432-1033.1991.tb16063.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2050146-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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