Article
Secretion and lipid association of human apolipoprotein E in Saccharomyces cerevisiae requires a host mutation in sterol esterification and uptake.
The Journal of biological chemistry - 5 Sept 1991
Sturley S L, Culbertson M R, Attie A D
Abstract excerpt
We have expressed a cDNA to human apolipoprotein E (apoE) in Saccharomyces cerevisiae. Secretion of apoE was achieved only by the use of a mutant (upc2) strain of yeast with the phenotype of enhanced uptake and intracellular esterification of exogenous cholesterol. Approximately 40 ng/ml apoE was...
Topics
- Apolipoproteins E
- Cholesterol
- Esterification
- Gene Expression Regulation, Fungal
- Humans
- Immunoblotting
- Lipid Metabolism
- Mutation
- Proprotein Convertases
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Serine Endopeptidases
- Subtilisins