Article
alpha-Factor pro-peptide N-linked oligosaccharides facilitate secretion of the insulin precursor in Saccharomyces cerevisiae.
Biotechnology and applied biochemistry - 1 Apr 1998
Kjeldsen T, Andersen A S, Hach M, Diers I, Nikolajsen J, Markussen J
Abstract excerpt
To evaluate the possible relationship between N-linked glycosylation of the Saccharomyces cerevisiae alpha-factor pro-peptide and transport of the alpha-factor pro-peptide/insulin precursor fusion protein through the Saccharomyces cerevisiae secretory pathway, we analysed secretion of insulin pre...
Topics
- Binding Sites
- Chromatography, High Pressure Liquid
- Electrophoresis, Gel, Pulsed-Field
- Glycosylation
- Insulin
- Insulin Secretion
- Mass Spectrometry
- Mating Factor
- Mutation
- Oligosaccharides
- Peptides
- Protein Precursors
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Temperature