Article
Effects of glycosylation on the secretion and enzyme activity of Mucor rennin, an aspartic proteinase of Mucor pusillus, produced by recombinant yeast.
The Journal of biological chemistry - 15 Aug 1990
Aikawa J, Yamashita T, Nishiyama M, Horinouchi S, Beppu T
Abstract excerpt
The Mucor rennin gene encoding a prepro form of the fungal aspartic proteinase from Mucor pusillus was expressed under the control of the yeast GAL7 promoter in Saccharomyces cerevisiae. The mature M. pusillus rennin secreted efficiently by yeast was a highly glycosylated protein. Analysis by a combination of site-directed mutagenesis of each of the three possible glycosylation sites and treatment of the secreted...
Topics
- Acetylglucosaminidase
- Base Sequence
- Chymosin
- Glycosylation
- Kinetics
- Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
- Models, Molecular
- Molecular Sequence Data
- Molecular Weight
- Mucor