U.v.-hypermutability of xeroderma pigmentosum cells demonstrated with a DNA-based mutation system.

Abstract excerpt

We have developed a DNA-based system, to detect mutations at restriction sites without any selection in culture. DNA is exhaustively digested with a restriction enzyme. Primers flanking a chosen site for this enzyme are used in the polymerase chain reaction (PCR). Only DNA molecules mutated at the chosen site are resistant to digestion and can serve as templates for the PCR. We have initially used this system to...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Cell Line
• Share to DNA Mutational Analysis