Article
U.v.-hypermutability of xeroderma pigmentosum cells demonstrated with a DNA-based mutation system.
Oncogene - 18 May 1995
Steingrimsdottir H, Beare D, Carr A M, Cole J, Lehmann A R
Abstract excerpt
We have developed a DNA-based system, to detect mutations at restriction sites without any selection in culture. DNA is exhaustively digested with a restriction enzyme. Primers flanking a chosen site for this enzyme are used in the polymerase chain reaction (PCR). Only DNA molecules mutated at th...
Topics
- Animals
- Base Sequence
- CHO Cells
- Cell Line
- Cricetinae
- DNA Mutational Analysis
- DNA, Neoplasm
- Ethyl Methanesulfonate
- Exons
- Genes, p53
- Genes, ras
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Restriction Mapping
- Xeroderma Pigmentosum