Article
Stable transformation of xeroderma pigmentosum group A cells with an XPA minigene restores normal DNA repair and mutagenesis of UV-treated plasmids.
Carcinogenesis - 1 Sept 1996
Myrand S P, Topping R S, States J C
Abstract excerpt
The ability of an XPA minigene construct to complement the DNA repair defect in xeroderma pigmentosum group A (XP-A) cells was demonstrated. XP-A cells (XP12BE-SV) were stably transformed with an XPA minigene linked to a neomycin resistance (neor) expression cassette. The G418-resistant clone XAN...
Topics
- Base Sequence
- Cell Line
- Cell Line, Transformed
- Chromosomes, Human, Pair 8
- DNA Primers
- DNA Repair
- DNA-Binding Proteins
- Dose-Response Relationship, Radiation
- Humans
- Kanamycin Kinase
- Molecular Sequence Data
- Mutagenesis
- Mutagenesis, Insertional
- Phenotype
- Phosphotransferases (Alcohol Group Acceptor)
- Plasmids
- Point Mutation
- Polymerase Chain Reaction