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Article

Host-dependent requirement for specific DnaA boxes for plasmid RK2 replication.

Molecular microbiology - 1 Aug 1999

Doran K S, Helinski D R, Konieczny I

Abstract excerpt

The replication origin of the broad-host-range plasmid RK2, oriV, contains four DnaA boxes, which bind the DnaA protein isolated from Escherichia coli. Using a transformation assay, mutational analysis of these boxes showed a differential requirement for replication in different Gram-negative bacteria. DnaA boxes 3 and 4 were required in E. coli and Pseudomonas putidabut not as strictly in Azotobacter vinelandii...

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Identifiers

PMID: 10417640
DOI: 10.1046/j.1365-2958.1999.01491.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Host-dependent requirement for specific DnaA boxes for plasmid RK2 replication.PMID 10417640Read ↗

A critical DnaA box directs the cooperative binding of the Escherichia coli DnaA protein to the plasmid RK2 replication origin.Semantic proximity Read ↗

Positioning and the specific sequence of each 13-mer motif are critical for activity of the plasmid RK2 replication origin.Semantic proximity Read ↗

A specific region in the N terminus of a replication initiation protein of plasmid RK2 is required for recruitment of Pseudomonas aeruginosa DnaB helicase to the plasmid origin.Semantic proximity Read ↗

Replication origin of the broad host range plasmid RK2. Positioning of various motifs is critical for initiation of replication.Semantic proximity Read ↗

Specific mutations within the AT-rich region of a plasmid replication origin affect either origin opening or helicase loading.Semantic proximity Read ↗

The box VII motif of Escherichia coli DnaA protein is required for DnaA oligomerization at the E. coli replication origin.Semantic proximity Read ↗

The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop mutations.Semantic proximity Read ↗

The host range of RK2 minimal replicon copy-up mutants is limited by species-specific differences in the maximum tolerable copy number.Semantic proximity Read ↗

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.Semantic proximity Read ↗

Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.Semantic proximity Read ↗

Modulation of pPS10 host range by plasmid-encoded RepA initiator protein.Semantic proximity Read ↗

Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2.Semantic proximity Read ↗

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